Sequence Studio

A quick sequence check, entirely in your browser

Paste a FASTA file (or drop one) to get GC-content landscapes, ORFs in all six frames, restriction maps, codon usage and translation — no upload, nothing leaves your machine.

How to use

1 · Load — pick an example, paste FASTA, or drag a .fasta file in.
2 · Explore — switch tabs: map, GC, ORFs, codons, tools.
3 · Export — download ORFs as FASTA or any table as CSV.

Sequence

Paste FASTA

Accession —

Length —

Topology —

GC content —

Annotated CDS —

Description —

Restriction enzymes

Minimum ORF length 100 aa

Sites are searched on the forward strand only (all listed enzymes are palindromic). Positions are the first base of the recognition sequence.

Restriction sites for the selected enzymes
Enzyme	Site	Count	Positions (1-based)

Minimum ORF length 100 aa

Selected ORF (for codon usage & translation)

Forward-strand ORFs are gold, reverse-strand ORFs are violet. The Match column names the annotated GenBank CDS an ORF corresponds to. Click a row or a bar to analyse that ORF.

Predicted open reading frames
Frame	Strand	Start	End	Length (aa)	Match	Protein (first 40 aa)

ORF

Standard genetic-code layout: rows are first·third base, columns are the second base. Darker = used more often in the selected ORF.

Codon usage

Amino-acid composition

Translation

Translate a selection

Enter 1-based start and end positions (inclusive), or leave blank for the whole record. Frames 1–3 read the forward strand; frames −1…−3 use the reverse complement.

Start End Frame

Reverse complement

Returns the reverse complement of the current record (or a selection).

Start End

Find motif (IUPAC codes)

Searches both strands for a motif using IUPAC ambiguity codes (e.g. R=A/G, Y=C/T, N=any, W=A/T, S=C/G, K=G/T, M=A/C, B=C/G/T, D=A/G/T, H=A/C/T, V=A/C/G). Overlapping hits are reported.

Motif

Data & sources

Bundled example sequences are unmodified NCBI Entrez E-utilities efetch responses, retrieved 2026-09-13. All analysis is computed locally in your browser; no data is uploaded.

Restriction-site recognition sequences are standard published motifs (New England Biolabs / REBASE). Motif search, translation and ORF prediction use the standard genetic code (table 11).